-Hemodynamic measurement in mouse, hamster, and rat models

A Millar pressure transducer catheter is advanced via the carotid artery into the left ventricle of the heart. Physiologyical data such as Maximum Pressure (mmHg), End Diastolic Pressure (mmHg), dP/dt (mmHG/s), NdP/dt (mmHg/s) Tau (msec) and 1/2Relaxation (msec) are recorded.

-Hemodynamic measurements with drug infusion challenge in mouse, hamster and rat models.

Hemodynamic measurements can be recorded while a drug is infused into the jugular vein.

-Blood flow measurement in rat and hamster models.

A technique to measure the relative blood flow to the heart using florescent microspheres. Functional data similar to hemodynamics may also be acquired from this method.

-Pressure volume loop measurements in Rat and Mouse

Using the (Millar Pressure Volume System) MPVS, cardiovascular pressure and volume signals can be plotted against each other in real time, generating the characteristic pressure-volume (P-V) loops that are an excellent illustration of the cardiac cycle in normal or diseased conditions of the living heart. With the MPVS, P-V loops may be captured during pharmacological, therapeutic, and hemodynamic interventions, allowing comprehensive evaluation of the fundamental mechanical properties of the heart. A single catheter with a high-fidelity pressure sensor and four electrodes is used to simultaneously measure left ventricular pressure and volume. To determine contractility and Emax a transient vessel occlusion is performed by isolating either the Inferior Vena Cava (to decrease preload) or the Aorta (to increase afterload).To convert measured volumed to absolute volume a saline bolus is injected into the jugular vein.

-Adult cardiac myocyte isolation from mouse, hamster and rat models.

 

-Aortic banding in Mouse and Rat

 

-Mouse and Rat MI

 

Core Utilization
The physiology cores primary staff member is David Wang.

The physiology calendar is made available to investigators who utilize the core services. By opening this calendar under shared calendars in outlook express, available times can be found and coordinated with the investigator's schedule.

Laboratory equipment usage is recorded on this calendar as are core personnel's schedule of availability. For more information on utilizing this calendar please click here.

Requests for core procedures and use of core equipment can be submitted by filling out the echo request form (for echoes) or the hemodynamic request form (for hemodynamics, hemodynamics with drug infusion, blood flow measurements, and PV loop measurements), or the cell mechanics request form (coming soon). The form should be emailed to coreB@usd.edu.

Space will be filled on a first come - first serve basis. You will be e-mailed confirmation for requested dates.

Please see David directly for any immediate needs.

If there is a physiology procedure that you would like to see offered through the physiology core that is not currently available please discuss these requests with Dr. Gerdes. He will make a determination if future training and availability is feasible.